Optimization of Conditions for Xylanase Production Using Aspergillus tubingensis Under Different Carbon Sources

Xylanases are hydrolytic enzymes with wide range of applications in food processing, bleaching pulp paper manufacturing industry, bio-conversion biomass wastes to fermentable sugars and enhancing nutrient digestibility animal feeds. The optimization growth conditions evaluation an appropriate substrate as carbon source among cassava peels, corn cobs, wheat bran rice husk on xylanase production by novel strain Aspergillus tubingensis under Solid State Fermentation (SSF) was investigated. fungal isolate identified based ribosomal RNA gene ITS sequencing analysis tubingensis. Results showed that Corn cobs had the highest four substrates. recorded value at pH 6.0 (107.97 U/g), after incubation period 72 hour (111.23 temperature 30oC (44.26 U/g) ratio 1:3 (45.68 U/g). optimum for were: 6.0, hours, concentration (4g per 12ml fermentation medium). yield activity (111.23±0.31 followed Rice (101.91±0.72 Wheat (89.30±1.16 Cassava peel (87.03±0.57 In conclusion, various agro residues were used sources, maximum activity. Various culture optimized using one factor a time method obtained hour, solid state fermentation. It is therefore suggested some other notable environmental factors influence metabolism-mediated yields like aeration, agitation, nitrogen metal ion requirement, inoculum size etc. should be enzyme. Keywords: Fermentation, bran,